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. Author manuscript; available in PMC: 2020 Jan 24.
Published in final edited form as: Cell. 2019 Jan 17;176(3):581–596.e18. doi: 10.1016/j.cell.2018.12.012

Figure 3. Linuron boosts IRE1α/XBP1 signaling via Sigmar1.

Figure 3.

A) Schematic of Sigmar1 protein domains. B) Co-immunoprecipitation of Sigmar1 and IRE1α in HEK293 cells. C) Western blot for NF-κB, P-IRE1α and XBP1 activation in neonatal murine astrocytes treated for 1 hour. n=3–7 per condition. One-way ANOVA, Sidak post-test. D) Xbp1 splicing in murine astrocytes activated for 24 hours (top). Representative images of agarose Xbp1 splicing gels (bottom). n=4–9 per condition. One-way ANOVA, Dunnett post-test. E) qPCR in neonatal murine astrocytes activated for 24 hours. n=7–16 per condition; n=4 for BD-1063 alone. One-way ANOVA, Tukey post-test. F) HEK293 luciferase reporter assay. n=4 per condition. One-way ANOVA, Tukey post-test on ln-normalized data.