TABLE 1.

Human Osteosarcoma Patient Information

Case #	Age at Primary Biopsy	Age at Metastasis Biopsy	Primary Tumor site	Metastasis Site	Biopsy Decalcification	Metastasis Decalcification	PD-L1 Metastatic Expression	PD-1⁺ TIL Metastatic Expression
1	7	9	R radius	L iliac bone and soft tissue	No	No	+	+
2	8	9	L distal femur	Mediastinum, thymus	Yes	No	+	+
3	14	18	R distal femur	Lung	No	No	+	−
4	12	14	R distal femur	Lung	Yes	No	+ +	+ +
5	14	15	L distal femur	Lung	No	Yes	−	−
6	13	14	R femur	Lung	No	No	−	+
7	12	15	L proximal tibia	Lung	Yes	No	−	+ +
8	10	10	L humerus	Lung	Yes	Yes	−	−
9	15	21	R femur	Lung	Yes	No	−	+
10	10	12	R shoulder	Lung	No	No	+	+ +
11	9	10	R distal femur	Lung	Yes	No	+ +	+ +
12	15	15	N/A	Lung	N/A	Yes	+	+
13	15	21	N/A	Lung	N/A	No	+	+
14	8	9	N/A	Lung	N/A	Yes	+	+
15	16	16	N/A	Lung	N/A	No	+ +	+ +
16	16	18	N/A	Lung	N/A	No	+ +	+

Human tissue used for evaluation of PD-L1 and PD-1 expression.

+ + indicates positive high level of expression (> 10 cells/field of view); +, positive low level of expression (< 10 cells/field of view); −, no positive cells per field of view; L, left; N/A, patient samples we obtained without matched primary tumor tissue; R, right.