Table 1.
Primary antibodies used
| Antigen | Immunogen | Host species | Reactivity | Source | Catalog# | RRID | Dillution | References |
|---|---|---|---|---|---|---|---|---|
| Casz1a | Human Casz1 peptide | Rabbit | N/A | Rockland | 600-401-B62S | AB_1961496 | 1:3000 | |
| Esrrg | AA 2–100 of human Esrrg | Mouse | Mouse, chicken | R&D systems | PP-H6812-00 | AB_2100280 | 1:1000 | |
| Evx1a | AA 1–192 | Rabbit | N/A | Dr. Martyn Goulding | N/A | N/A | 1:300 | (Moran-Rivard et al., 2001) |
| Evx2 | AA 92–102 of mouse Evx2 | Guinea pig | Mouse | Dr. Ryuichi Shirasaki | N/A | N/A | 1:6 000 | (Inamata and Shirasaki, 2014) |
| FoxP2 | A peptide near N-terminus of human Foxp2 | Goat | Mouse, chicken | Santa Cruz Biotechnology | sc-21069 | AB_2107124 | 1:500 | |
| Lbx1 | Full-length mouse Lbx1 | Guinea pig | Mouse, chicken | Dr. Thomas Müller | N/A | N/A | 1:30,000 | (Müller et al., 2002) |
| Lhx1/5 | AA 1–360 of rat Lhx5 | Mouse | Mouse, chicken | DSHB | 4F2 | AB_531784 | 1:15 | (Tsuchida et al., 1994) |
| Maf | AA 150–200 of mouse Maf | Rabbit | Mouse, chicken | Bethyl Laboratories | A300-613 | N/A | 1:2000 | |
| Myca | AA 408–439 of human Myc | Mouse | N/A | DSHB | 9E 10 | AB_2266850 | N/A | |
| Myca | Full-length human Myc | Rabbit | N/A | Millipore | 06-340 | AB_310106 | 1:1000 | |
| Myca | AA ∼1–100 of human Myc | Rabbit | N/A | Abcam | ab32072 (Y69) | AB_731658 | 1:18,000 | |
| Onecut1 | Mix of AA 11–53 and 63–81 of mouse Onecut1 | Guinea pig | Mouse | Dr. Frédéric Clotman | N/A | N/A | 1:5000 | (Espana and Clotman, 2012) |
| Onecut1 | AA 11–110 of human Onecut1 | Rabbit | Mouse, chicken | Santa Cruz Biotechnology | sc-13050 | AB_2251852 | 1:300 | |
| Onecut2 | AA 185–326 of human Onecut2 | Sheep | Mouse | R&D systems | AF6294 | AB_10640365 | 1:500 | |
| Onecut3 | AA 23–333 of mouse Onecut3 | Guinea pig | Mouse | Dr. Frédéric Clotman | N/A | N/A | 1:6000 | (Pierreux et al., 2004) |
| Phox2b | A peptide near N-terminus of human Phox2b | Goat | Mouse | Santa Cruz Biotechnology | sc-13224 | AB_2251852 | 1:1000 | |
| Phox2b | N/A | Rabbit | Chicken (mouse untested) | Dr. Jean-François Brunet | N/A | N/A | 1:20,000 | Unpublished |
| Pou3f1 | A peptide near C-terminus of human Pou3f1 | Goat | Mouse | Santa Cruz Biotechnology | sc-11661 | AB_2268536 | 1:500 |
a
Antibodies that stained most cells in the hindbrain, or had excessive background signal. AA, Amino acids; DSHB, Developmental Studies Hybridoma Bank; N/A, not available.