KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-OPN4 antiserum | Salk Institute, Pulivarthy et al.,2007 | RRID: AB_2571553 |
| Mouse monoclonal anti b-arrestin 1/2 | Santa Cruz | sc-74591, |
| Normal rabbit anti-IgG | Santa Cruz | sc-2027, RRID: AB_737197 |
| Rabbit polyclonal anti-TBP | Santa Cruz | sc-273 |
| Alexa Fluor 680 conjugate anti-Rabbit IgG | Thermo Fisher | A-10043 |
| Alexa Fluor 680 conjugate anti-mouse IgG | Thermo Fisher | A-21058 |
| Bacterial and Virus Strains | ||
| pAAV2/2-MSC8-mOpn4 WT-1d4tag | Salk Institute virus core, Mure et al., 2016 | N/A |
| pAAV2/2-MSC8-mOpn4 397truncation-1d4tag | Salk Institute virus core, Mure et al., 2016 | N/A |
| pAAV2/2-MSC8-mOpn4 9Amutation-1d4tag | Salk Institute virus core, Mure et al., 2016 | N/A |
| AAV2-hSyn-mOpn4-F2A-mβArr1a-flag | Salk Institute virus core, this study | N/A |
| AAV2-hSyn-mOpn4-F2A-mβArr2-flag | Salk Institute virus core, this study | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 11 cis retinal | National Eye Institute | https://nei.nih.gov/funding/11_cis_retinal |
| Fluo-4 AM | Molecular Probes | F14201 |
| Critical Commercial Assays | ||
| PathHunter® β-Arrestin Assays | DiscoverX | https://www.discoverx.com/arrestin |
| Experimental Models: Cell Lines | ||
| CHO cells stably expressing human melanopsin (CHOOpn4) | Jones et al., 2013 | N/A |
| Experimental Models: Organisms/Strains | ||
| Arrb1 KO | The Jackson Laboratory (Robert Lefkowitz) | N/A |
| Arrb1 KO | The Jackson Laboratory (Robert Lefkowitz) | N/A |
| rd (C3H/HeJ) | The Jackson Laboratory | N/A |
| rd;b1 | This study | N/A |
| rd;b2 | This study | N/A |
| Opn4cre/cre | This study | RRID: MGI_3798479 |
| rd;Opn4cre/cre | This study | RRID:MGI_5754375 |
| Software and Algorithms | ||
| Prism | GraphPad | RRID: SCR_015807, https://www.graphpad.com |
| MATLAB | MathWorks | RRID: SCR_001622, https://www.mathworks.com/products/matlab.html |
| Plexon Offline Sorter | Plexon | RRID:SCR_000012, https://plexon.com/products/offline-sorter/ |
| NeuroExplorer | Plexon | RRID:SCR_001818, http://plexon.com/products/neuroexplorer |
| MC Rack | Multichannelsystems | RRID:SCR_014955, https://www.multichannelsystems.com/software/mc-rack |
| LI-COR Image Studio | LI-COR Biosciences | RRID: SCR_015795, https://www.licor.com/bio/products/software/image_studio/ |