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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Cell Rep. 2018 Nov 27;25(9):2497–2509.e4. doi: 10.1016/j.celrep.2018.11.008

Figure 5. βArr2 Is Required for Signal Termination, while bArr1 Participates in Receptor Recycling.

Figure 5.

(A and B) ipRGCs responses profiles to light (MEA, nm, 5 × 1012 photons/cm2/s, 100 ms, 1 s, 10 s, and 60 s, 5 repetitions each) in retinas from rd;βarr1–/– (A) and rd;βarr2–/– (B) mice.

(C) rd;βarr1–/– ipRGCs display strong adaptation when repetitively stimulated for 1 min (slopes different from 0: rd, **p = 0.005, rd;βArr1, ***p = 0.0002, rd;βArr2, p = 0.52, ns; slopes different from rd: rd;bArr1, ***p = 0.0007, rd;βArr2, **p = 0.004).

(D) Adaptation rate (relative response change from one stimulation to the other, average ± SEM) observed in the β-arrestin-deficient retina model (rd compared to rd;βArr1 and rd;βArr2) and β-arrestin overexpression model (AAV Opn4WT compared to AAV Opn4WT-F2A-bArr1 or 2) in response to 5 repetitions of an identical 1-min-long light stimulus (MEA, 480 nm, 5 × 1012 photons/cm2/s) (t test, A2 versus A2-F2A-βarr2,***p = 1.9 3 10 5; A2 versus A2-F2A-βarr1, ***p = 5.× 3 10 10; rd versus rd;βarr1–/–, ***p = 5.5 × 10 5; rd versus rd;βarr2–/–, **p = 0.003).

(E) LRAM from rd;Opn4–/– mice retinas transduced with Opn4WT (n = 57), Opn4WT-F2A-βArr1 (n = 45), and Opn4WT-F2A-βArr2 (n = 111) (100 ms, 1s, 10 s, and 1 min, 480 nm, 5 × 1012 photons/ cm2/s).