Table 1.
Nuclease activity of LbCpf1 in Sprague–Dawley rat embryos and generation of Apoe gene knockout rats.
| crRNA + Cpf1 mRNA (ng/μL) | Injected zygotes | Survived & transferred (%)a | Newborns (%)b | Mutants (%b, %c) | Mosaicism frequency (%)d |
|---|---|---|---|---|---|
| 100 + 50 | 42 | 41 (97.6) | 7 (17.1) | 5 (12.2, 71.4) | 1 (20) |
| 50 + 50 | 77 | 77 (100) | 11 (14.3) | 2 (2.6, 18.2) | 0 (0) |
Percentages were calculated using the number in each column as the numerator and the numbers of ainjected zygotes, bsurviving and transferred embryos, cnewborns, or dmutants as the denominator.