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. 2019 Feb 22;9:2628. doi: 10.1038/s41598-019-38732-2

Table 1.

Nuclease activity of LbCpf1 in Sprague–Dawley rat embryos and generation of Apoe gene knockout rats.

crRNA + Cpf1 mRNA (ng/μL) Injected zygotes Survived & transferred (%)a Newborns (%)b Mutants (%b, %c) Mosaicism frequency (%)d
100 + 50 42 41 (97.6) 7 (17.1) 5 (12.2, 71.4) 1 (20)
50 + 50 77 77 (100) 11 (14.3) 2 (2.6, 18.2) 0 (0)

Percentages were calculated using the number in each column as the numerator and the numbers of ainjected zygotes, bsurviving and transferred embryos, cnewborns, or dmutants as the denominator.