Figure 4.
Recruitment and Adaptation of Treg Cells to the Tumor Environment Recapitulates Steady-State Migration
(A) Melanoma induction strategy and sampled tissues.
(B) t-SNE depicting Treg and Tmem cells from tumor and steady-state skin, draining brachial lymph nodes, and spleen.
(C) Differential expression between skin and tumor Treg cells. Treg cells classified as cycling were excluded.
(D) (top) Latent variables found with MRD-BGPLVM representing cell cycle (LV5) and non-lymphoid tissue recruitment/adaptation of Treg cells (LV9). (bottom) Distribution of cells based on Tissue and Condition and Cell-Cycle phase along the recruitment trajectory.
(E) Difference in activation time (t0) of genes in control and tumor. Genes are classified as being markers of skin, lymph node, cell cycle, or other. Colored points show mean ± mean SE for each group. Vertical dashed lines represent the mean ± SE for all t0 values. t test between control and melanoma t0 indicates no change (p value = 0.2631), with t0 values having a Spearman correlation coefficient of 0.65 between both conditions. See also Figure S4.