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. 2019 Jan 25;2(1):e201800282. doi: 10.26508/lsa.201800282

Figure 2. STING activation leads to the inhibition of the mTORC1 pathway.

Figure 2.

(A, B) Western blot analysis of the indicated phosphorylated proteins in CD4+ T cells from Sting+/+ or Sting−/− mice upon stimulation with anti-CD3/CD28 Abs with or without cGAMP for 24 h. (C) FACS analysis of indicated surface molecules on CD4+ T cells upon stimulation with anti-CD3/CD28 Abs with or without cGAMP for 24 h. (D) RNA-seq data of the expression of lipid synthesis–related genes in CD4+ T cells upon stimulation with anti-CD3/CD28 Abs with or without cGAMP for 24 h. (E) Quantification of total cholesterol ester (upper) and total DAG (lower) in CD4+ T cells upon stimulation with anti-CD3/CD28 Abs with or without cGAMP for 24 h, assessed by LC-MS. Data are the mean from triplicate ± SD (E). Data are representative of at least three independent experiments (A–C). (E) *P < 0.05, t test (compared with that with anti-CD3/28 alone).