Figure 3.
Characterization of RBPJc.A611T/p.H204L mutation–reactive T-cell response. 4062-TILs were cocultured with RBPJc.A611T/p. H204L 25-mer peptide-pulsed autologous B cells that were preincubated with vehicle or the indicated HLA-blocking antibody. A, HLA-blocking assay demonstrating MHC class II and HLA-DR restriction. B, The HLA-DR restriction element was identified by coculturing 4062-TILs with autologous EBV-B cells or allogeneic EBV-B cells partially matched at theHLA-DR locus. The 4062-TILs CD4+ T-cell response was shown to be HLADRB1*15:01 restricted. C, 4062-TILs were cocultured with autologous B cells pulsed with RBPJc.A611T/p.H204L 25-mer peptide or the indicated truncated peptides. *, location of mutated amino acid at position 13, and the predicted peptide binding core sequence is underlined. All data are based on IFNγ ELISPOT and representative of at least three independent experiments. Error bars, SEM.