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. Author manuscript; available in PMC: 2019 Jul 14.
Published in final edited form as: Nat Immunol. 2019 Jan 14;20(2):163–172. doi: 10.1038/s41590-018-0276-y

Figure 2. Reference datasets reveal heterogeneity of macrophage subtypes in lung fibrosis.

Figure 2.

a-c, t-SNE plots of single-cell suspensions from mouse whole lung sequenced by Drop-seq color-coded by condition (a); annotated by SingleR using the ImmGen database as reference (b); and annotated by SingleR with lung-specific myeloid datasets26,27 (c). Data shown pool replicates (n=3 biologically independent mice for bleomycin-injured and n=6 biologically independent mice for healthy control). d, Quantification of similarity in gene expression of individual cells to bulk RNA-seq profiles of alveolar macrophages (AM) and CD11c+ interstitial macrophages (IM)27.