Skip to main content
. 2019 Jan 10;10:131. doi: 10.1038/s41467-018-08027-7

Table 1.

Summary of binary (PROTAC:p38δ or PROTAC:VHL) and ternary (p38δ:PROTAC:VHL) affinity and kinetic measurements

SJFδ SJFα
PROTAC:p38δ Kd 550 nM 500 nM
PROTAC:VHL Kd 3500 nM 5000 nM
p38δ:PROTAC:VHL Kd 436 nM 1200 nM
p38δ:PROTAC:VHL koff 0.018 s−1 0.072 s−1
p38δ:PROTAC:VHL kon 3.96 × 104 M−1 s−1 7.01 × 104 M−1 s−1
p38δ:PROTAC:VHL t1/2 38 s 8 s

The last protein listed in each list above represents that which was immobilized on the SPR instrument. See Methods section for further descriptions