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. Author manuscript; available in PMC: 2018 Dec 20.
Published in final edited form as: Mol Immunol. 2016 May 6;74:82–95. doi: 10.1016/j.molimm.2016.04.013

Table 1.

Summary of TCR CDR3β sequences of memory CD4+ T cells from PaLN of NOD mice

Mouse
#		 Total
sequencesa 		Productive
sequencesb 		Out of frame
sequencesc 		Unique
clonotypesd 		Entropye
Prediabetic 1 1,383,340 1,290,284 91,804 4,684 0.910387
2 853,941 802,119 51,371 6,140 0.922921
3 1,080,711 967,175 112,840 6,277 0.925919
4 609,154 564,970 43,611 13,515 0.928411
5 1,241,670 1,156,949 83,220 17,673 0.918004
6 1,730,239 1,583,074 145,314 36,695 0.918892
Total n = 6 6,899,055 6,364,571 528,160 84,984
Diabetic 7 1,218,928 1,116,981 100,243 22,726 0.928054
8 1,223,723 1,139,121 82,845 20,199 0.930243
9 2,004,499 1,871,072 131,793 25,899 0.893506
10 587,048 548,887 37,683 2,010 0.901663
11 1,815,300 1,644,275 167,816 16,374 0.924445
12 910,306 837,474 72,023 11,434 0.931586
Total n = 6 7,759,804 7,157,810 592,403 98,642
a

Number of nucleotide sequences obtained.

b

Number of in-frame, read-through nucleotide sequences.

c

Number of out-of-frame nucleotide sequences

d

Number of unique amino acid sequences encoded by in-frame, read-through nucleotide sequences. Each unique amino acid sequence identified one unique clonotype.

e

Shannon’s entropy was used to quantify diversity of the TCRβ repertoire.