KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CEP290 | Proteintech Group | Cat#22490–1-AP; RRID: AB_10973679 |
| Anti-mouse CEP290 | A Swaroop (PI) (Chang et al., 2006) | N/A |
| Anti-mouse S-OPSIN | T Li (PI) (Sun et al., 2010) | N/A |
| Anti-mouse M-OPSIN | Millipore | Cat#AB5405; RRID: AB_177456 |
| Anti-mouse RHODOPSIN | T Li (PI) (Li et al., 1996) | N/A |
| Anti-mouse GNAT2 | Santa Cruz | Cat#sc-390; RRID: AB_2279097 |
| Anti-mouse β-Actin | Sigma-Aldrich | Cat#A5441; RRID: AB_476744 |
| Anti-mouse Cone PDE | T Li (PI) (Sun et al., 2010) | N/A |
| Anti-mouse Rod PDE | T Li (PI) (Sun et al., 2010) | N/A |
| Anti-mouse ROOTLETIN | T Li (PI) (Yang et al., 2002) | N/A |
| Anti-mouse RPGR | T Li (PI) (Hong and Li, 2002) | N/A |
| Anti-mouse AHI1 | Abcam | Cat#Ab93386; RRID: AB_10561623 |
| Anti-MYC Tag | Cell Signaling | Cat#2276; RRID: AB_331783 |
| Anti-HA Tag | Cell Signaling | Cat#2367; RRID: AB_331789 |
| Anti-mouse RPGRIP1 | T Li (PI) (Hong et al., 2001) | N/A |
| Anti-FLAG Tag | Sigma-Aldrich | Cat#A2220; RRID: AB_1006303 |
| Anti-FLAG Tag | Cell Signaling | Cat#2368; RRID: AB_2217020 |
| HRP-conjugated Donkey Anti-Rabbit IgG (H+L); AffiniPure |
Jackson ImmunoResearch | Cat#711035152; RRID: AB_10015282 |
| HRP-conjugated Donkey Anti-Mouse IgG (H+L); AffiniPure |
Jackson ImmunoResearch | Cat#715035150; RRID: AB_2340770 |
| Alexa Fluor 488 Donkey anti-Chicken IgY (IgG) (H+L); AffiniPure |
Jackson ImmunoResearch | Cat#703545155; RRID: AB_2340375 |
| Alexa Fluor 488 Donkey anti-Rabbit IgG (H+L) | Invitrogen | Cat#A21206; RRID: AB_141708 |
| Alexa Fluor 488 Donkey anti-Mouse IgG (H+L) | Invitrogen | Cat#A21202; RRID: AB_141607 |
| Alexa Fluor 568 Donkey anti-Mouse IgG (H+L) | Invitrogen | Cat#A10037; RRID: AB_2534013 |
| Alexa Fluor 568 Donkey anti-Rabbit IgG (H+L) | Invitrogen | Cat#A10042; RRID: AB_2534017 |
| Alexa Fluor 594 Lectin PNA from Arachis hypogaea | Invitrogen | Cat#L32459 |
| DAPI | Invitrogen | Cat#D1306; RRID: AB_2629482 |
| Anti-mouse IgG for IP (HRP) (veriblot antibody) | Abcam | Cat#131368 |
| VeriBlot for IP Detection Reagent (HRP) | Abcam | Cat#131366 |
| Bacterial and Virus Strains | ||
| NEB-10β | New England Biolab | Cat#C3019H |
| AAV8-CMV-MYC-MYO tail (mCep290) | This paper | N/A |
| AAV8-CMV-N-term (mCep290) | This paper | N/A |
| AAV8-CMV-C-term (mCep290) | This paper | N/A |
| AAV8-CMV-DSD (mCep290) | This paper | N/A |
| AAV8-CMV-HA-MYO tail (mCep290) | This paper | N/A |
| AAV2-CMV-MYC-MYO tail (mCep290) | This paper | N/A |
| AAV2-CMV-EGFP | This paper | N/A |
| AAV8-RK-Cas9 | Zhijian Wu (PI) (Yu et al., 2017) | N/A |
| AAV8-CMV-MYC-MYO tail (mCep290)-U6- sgRNA_EGFP |
This paper | N/A |
| AAV8-CMV-MYC-MYO tail (mCep290)-U6- sgRNA_mCep290 |
This paper | N/A |
| Critical Commercial Assays/Chemicals | ||
| Co-Immunoprecipitation kit | ActiveMotif, | Cat#54002 |
| Fluoromount-G | SouthernBiotech | Cat#0100–01 |
| QIAGEN Miniprep kit | QIAGEN | Cat#27106 |
| QIAGEN Hi-Speed Maxi kit | QIAGEN | Cat#12662 |
| Lipofectamin 3000 | ThermoFisher Scientific | Cat#L3000001 |
| SuperSignal west Pico | ThermoFisher Scientific | Cat#34577 |
| SuperSignal west Femto | ThermoFisher Scientific | Cat#34094 |
| Experimental Models: Cell Lines | ||
| HEK293 cell line | ATCC | CRL-1573 |
| Mouse embryonic fibroblasts | This paper | N/A |
| Mouse iPSCs | A Swaroop (PI) (Chen et al., 2016) | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | JAX stock #000664 |
| Mouse: B6.Cg-Cep290rd16/Boc | The Jackson Laboratory | JAX stock #012283 |
| Mouse: Cep290rd16/rd16;Nrl—/— | A Swaroop (PI) (Cideciyan et al., 2011) | N/A |
| Mouse: B6;129-Nrltm1Asw/J (or Nrl—/—) | The Jackson Laboratory | JAX stock #021152 |
| Mouse: Cep290+/—;Nrl—/— | This paper | N/A |
| Oligonucleotides | ||
| Primers for Cep290 fragments, see Table S1 | This paper | N/A |
| CMV-probe: 6FAM-TCCAAAATGTCGTAACAA CT–MGBNFQ |
This paper | N/A |
| CMV F: TGGGAGTTTGTTTTGCACCAA | This paper | N/A |
| CMV R: CGCCTACCGCCCATTTG | This paper | N/A |
| sgRNA Cep290: CCAGGAAGTAGAGCTCGCTT | This paper | N/A |
| sgRNA EGFP: CGCGCCGAGGTGAAGTTCGA | Z Wu (PI) (Yu et al., 2017) | N/A |
| Software and Algorithms | ||
| GraphPad prism | GraphPad software | https://www.graphpad.com/scientific-software/prism/ |
| Espion software | Diagnosys LLC | http://diagnosysllc.com/products/ |
| OptoMotry© software (ver14) | Cerebral Mechanics | http://www.cerebralmechanics.com/ |