Figure 4.
TNT Formation and Mitochondrial Transfer from iPSC-MSCs Labeled with MitoTracker into BEAS-2B Cells
(A–C) TNT-like structures were observed between normal BEAS-2B cells (A), between damaged BEAS-2B cells (B), and between normal iPSC-MSCs (C).
(D) TNTs were observed connecting iPSC-MSCs with normal BEAS-2B cells at 24 hr after co-culture.
(E) More TNTs were observed between iPSC-MSCs and CoCl2-damaged BEAS-2B cells induced 24 hr after co-culture. TNTs were formed between normal BEAS-2B cells (b), damaged BEAS-2B cells (d), and normal N1-iPSC-MSCs (e); between normal N1-iPSC-MSCs and normal BEAS-2B cells (f, g, k); and between normal N1-iPSC-MSCs and damaged BEAS-2B cells (m, n, p, q, r, s, u).
(F) iPSC-MSCs with MitoTracker Red-labeled mitochondria (red) were co-cultured with BEAS-2B cells (green). Mitochondrial transfer was detectable as early as 4 hr after co-culture. More mitochondria were transferred into BEAS-2B cells from iPSC-MSCs over time. BEAS-2B cells were labeled using CFSE (green) in (D)–(F). Experiments were carried out in triplicates.
CFSE, 5(6)-carboxyfluorescein N-hydroxysuccinimidyl ester; iPSC-MSC, induced pluripotent stem cell-derived mesenchymal stem cells; OVA, ovalbumin; TNT, tunneling nanotube. See also Figure S2.