Figure 4.
RGS7 regulates sIPSC kinetics. A, Schematic of the experimental strategy. The sIPSCs were recorded in CA1 neurons while evoking GABA release from interneurons by placing the stimulation electrode on the border of stratum radiatum and stratum lacunosum moleculare along the apical dendrites. B, Representative normalized traces of sIPSCs evoked by 400 μA biphasic stimulating current from WT (RGS7+/+ littermates), RGS7 KO, and Gβ5 KO hippocampal slices (left). Inset, Peak region of sIPSCs (right). C, Average sIPSC amplitudes in WT, RGS7 KO, and Gβ5 KO. D, Deactivation time constant determined via single-exponential fitting of response traces in RGS7 KO and Gβ5 KO versus WT (***p < 0.001 vs WT, one-way ANOVA with Bonferroni's post tests, n = 9–10). E, Response onset timing determined as time to peak (TTP) values in RGS7 KO and Gβ5 KO (***p < 0.001 DKO vs WT).