Fig. 7.
Comparison of model output and experimental (siRNA) data for BMAL1::LUC and PER2::LUC oscillations in both U2-OS and 3T3 cells. Simulated (A) amplitude and (B) baseline of BMAL1::LUC oscillations and relevant siRNA data (U2-OS, pattern fill; 3T3, white fill). Simulated (C) amplitude and (D) baseline of PER2::LUC oscillations compared with relevant siRNA. Data are represented as mean ± SEM and normalized with respect to control (NEGsi) condition while applying the naming convention of U2-OS. Simulations are performed under conditions where SIRT1 deacetylates both PER2 and PGC1α but not BMAL1. Correlation analysis suggests that the effect of SIRT1 on these targets is critical for recapitulating the relevant siRNA data for both BMAL1/PER2 LUC oscillations. Correlation coefficient for the amplitude and baseline of BMAL1::LUC oscillations is 0.9 (P value = 0.001) and 0.8 (P value = 0.03), respectively. Correlation coefficient for the amplitude and baseline of PER2::LUC oscillations is 0.8 (P value = 0.01) and 0.9 (P value < 0.001), respectively. Note that (i) the U2-OS PER2si data have been excluded from the estimation of the correlation coefficient and (ii) gene names are represented in the uppercase italics naming convention (i.e., for genes of human origin) only for simplifying this figure’s representation.