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. Author manuscript; available in PMC: 2019 Aug 2.
Published in final edited form as: Cell Stem Cell. 2018 Aug 2;23(2):239–251.e6. doi: 10.1016/j.stem.2018.07.009

Figure 3. AxD Astrocytes Cause Myelination Defect in a 3D Nanofiber Culture System.

Figure 3.

(A and C) Representative images of MBP immunostaining in a 3D nanofiber culture system that include GFAP-GFP-sorted control vs AxD (A), or AxD999-CR vs AxD999 (C) astrocytes (green) and oligodendrocytes matured from I90 hiPSC-derived OPCs. Scale bar: 50 μm. (B and D) Representative images of MBP immunostaining and alignment with nanofibers in control vs AxD (B), or AxD999-CR vs AxD999 (D) co-cultures. Scale bar: 100 μm. Higher magnification images are shown underneath panel B to highlight the alignment of the MBP segments with nanofibers. Scale bar: 20 μm. (E-G) Fold change in MBP+ cell number (#) (E), MBP+ area (F), and MBP+ segment length (G) in nanofiber-based co-culture of OPCs with astrocytes. Error bars are SE of the means, n=3 experimental repeats, *p<0.05, **p<0.01, and ***p<0.001 by one-way ANOVA for panels E-G. See also Figure S3.