Figure 3. SAMMSON interacts with XRN2 and CARF.
(a) SAMMSON (but not TBP or UBC) is specifically recovered by RAP after UV crosslinking SK-MEL-28 cells. Representative image of three independent experiments. (b) SAMMSON and HRPT pulldown in native conditions (using two sets of 48 biotinylated probes recognizing mature transcripts, p) and western blotting in SK-MEL-28 cells. Representative image of three independent experiments. (c) SAMMSON and HRPT pulldown in native conditions (using biotinylated probes, p) and western blotting in mouse p38 cells. Representative image of three independent experiments. (d) MALAT1 (but not SAMMSON or HPRT) is recovered by RIP using an SRSF1- specific antibody as measured by RT-qPCR in SK-MEL-28 cells. Error bars represent mean ± s.e.m.; n=3. (e) Western blot confirming enrichment of SRSF1 following RIP with an anti- SRSF1 antibody in SK-MEL-28 cells. Representative image of three independent experiments. (f) SAMMSON and TERRA are recovered by RIP using an XRN2-specific antibody as measured by RT-qPCR in SK-MEL-28 cells. Error bars represent mean ± s.e.m.; n=3. (g) Western blot confirming enrichment of XRN2 (and CARF) following RIP with an anti-XRN2 antibody in SK-MEL-28 cells. Representative image of three independent experiments. (h) SAMMSON and LINC00698 are recovered by RIP using a CARF-specific antibody as measured by RT-qPCR in SK-MEL-28 cells. Error bars represent mean ± s.e.m.; n=3. (i) Western blot confirming enrichment of CARF (and XRN2) following RIP with an anti-CARF antibody in SK-MEL-28 cells. Representative image of three independent experiments. Uncropped gel images are shown in Supplementary Data Set 1.