Figure 2. Interneuron activity levels predict cell death.
a, Schematic of experimental design. b, Total number of MGE/POA interneurons in layer 2/3 S1BF of Nkx2-1-Cre;RCLtdTomato mice (n = 3 animals for all ages). Data is shown as mean ± SEM. c, ROI imaged at P7 (left) and P8 (right). d, Raster plots showing the occurrence of calcium events at P7 for the four neurons shown in (c). e, Box plots illustrating event rates for P7 interneurons that live past P8 (magenta) and interneurons that die by P8. Two-sided Mann-Whitney test, p = 0.03; n = 18 for cells that die at P8 and 153 for cells that live beyond P8, from 3 different pups. f, ROC analysis showing the ability of P7 event rates to discriminate between cells that die by P8 and cells that live past P8, AUC (area under the curve) = 0.65, *p = 0.025. Scale bar, 15 µm.