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Experimental scheme depicting transduction on fresh or once-thawed CD34+ cells and the single or double freeze–thaw events after transduction. (A) Freshly transduced cells manipulated right after CliniMACS immunomagnetic separation. (B) Cells cryopreserved as unmanipulated after CliniMACS immunomagnetic separation and thawed for transduction. Pre-stim, pre-stimulation; CFU, colony-forming unit.
