FIGURE 8.
Uncoupling STING-mediated NFkB activation and IRF3 activation. BMDCs (A) or BMDMs (B) from C57BL/6 mice were treated with Bx-795 (1 μM), MG-132 (4 μg/ml), or DMSO (mock) for 2 h. Cells were then activated by CDG (10 μg/ml) for 4 h. Nuclear fractions were isolated and processed as in Fig. 7 (n = 3). BMDCs (C) or BMDMs (D) were treated with Bx-795 (1 μM) or DMSO (mock) as in (A) and (B). Cells were then activated by CDG (10 μg/ml) for the indicated time. WCLs were run on SDS-PAGE gel and probed with the indicated Abs (n = 3). BMDCs (E) or BMDMs (F) were treated with MG-132 (4 μg/ml) or DMSO (mock) as in (A) and (B). Cells were then activated by CDG (10 μg/ml) for the indicated time. WCLs were run on SDS-PAGE gel and probed with the indicated Abs (n = 3). n.s, Nonspecific.