FIGURE 4.
IFN-I signaling is dispensable for CDG-induced cytokine and chemokine production in vivo. (A) WT, STING−/−, and IFNAR−/− mice were given CDG (10 μg) or PBS i.n. Cytokine production was measured in BALF as in Fig. 2 (n = 3). Data are mean ± SE from three independent experiments. (B) Live cells from BALF were analyzed by FACScan with the indicated Abs as in Fig. 2 (n = 3). (C) BMDCs from WTand IFNAR−/− μiχε were activated by CDG (10 μg/ml) or medium alone (mock) for 18 h. Cells were stained with Abs against CD80 and CD86. Live cells were analyzed by flow cytometry (n = 3). *p, 0.05. n.s., Nonsignificant.