| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat Anti-GFP antibody (FITC) | Abcam | Cat# ab6662 RRID:AB_305635 |
| Rabbit-anti-mCherry | Abcam | Cat# ab167453 RRID:AB_2571870 |
| Alexa Fluor® 647 recombinant rabbit monoclonal anti-Neuronal Nuclei (NeuN) | Abcam | Cat# ab190565 RRID: EPR12763 |
| Chicken polyclonal to Tyrosine Hydroxylase antibody | Abcam | Cat# ab76442 RRID: AB_1524535 |
| Mouse anti-TH | ImmunoStar | Cat# 22941 RRID: AB_10731005 |
| Goat polyclonal to CGRP | Abcam | Cat# ab36001 RRID:AB_725807 |
| Alexa Fluor® 488 conjugated affinipure goat anti-chicken IgY (H&L) | Abcam | Cat# ab150169 RRID:AB_2636803 |
| Anti-c-Fos Rabbit polyclonal to c-Fos | Abcam | Cat# ab 190289 RRID: AB_2231989 |
| TRITC-conjugated affinipure Goat anti-Rabbit IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 111-025-144 RRID:AB_2337932 |
| FITC-conjugated affinipure goat anti-rabbit IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 111-095-144 RRID:AB_2337978 |
| FITC-conjugated affinipure Donkey Anti-Goat IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 705-095-147 RRID:AB_2340401 |
| TRITC-conjugated affinipure Donkey Anti-Goat IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 705-025-147 RRID:AB_2340389 |
| Biotinylated Goat Anti-Rabbit IgG antibody | Vector Laboratories | Cat# BA-1000 RRID:AB_2313606 |
| Goat anti-Phaseolus Vulgaris Agglutinin (E+L), Unconjugated | Vector Laboratories | Cat# AS-2224 RRID: AB_10000080 |
| Rabbit anti-PHA-L | Dako | Cat# B275 RRID: AB_2315137 |
| Rabbit anti-FG | Chemicon®, Millipore | Cat# AB153-I RRID: AB_2632408 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| FluoroGold (FG) | Manufacturer: Fluorochrome Inc; Purchased from Fisher scientific | Cat# NC0560981 |
| PHA-L | Vector | Cat# L-1110 |
| Red RetroBeads | Lumafluor | N/A |
| CCK-SAP | Advanced Targeting Systems | Cat # IT-31 |
| SAP | Advanced Targeting Systems | Cat # IT-21 |
| CCK8 | AnaSpec | Cat # AS-20739 |
| Clozapine N-oxide (CNO) | Enzo Life Sciences | Cat # BML-NS105–0025 |
| Clozapine | Sigma | Cat # C6305–25MG |
| cis-(Z)-Flupenthixol dihydrochloride | Sigma | Cat # F114 |
| Metroclopramide Hydrochloride | Sigma | Cat # M0763–10G |
| Critical Commercial Assays | ||
| VECTASTAIN Elite ABC HRP Kit (Peroxidase, Standard) | Vector Laboratories | Cat# PK-6100 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | JAX: 000664 |
| Mouse: Slc32a1tm2(cre)Lowl/J | The Jackson Laboratory | JAX: 016962 |
| Mouse: Slc17a6tm2(cre)Lowl/J | The Jackson Laboratory | JAX: 016963 |
| Mouse: Slc6a3tm1.1(cre)Bkmn/J | The Jackson Laboratory | JAX: 006660 |
| Mouse: Agrptm1(cre)Low | The Jackson Laboratory | JAX: 012899 |
| Mouse: B6;129S-Gt(ROSA)26Sortm34.1(CAG-Syp/td Tomato)Hze/J | The Jackson Laboratory | JAX: 012570 |
| B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | The Jackson Laboratory | JAX: 007914 |
| Recombinant DNA | ||
| AAVrg-pmSyn1-EBFP-Cre | Dr. Zeng-Addgene AAV Viral Service | 51507 |
| H129 | NIH Center for Neuroanatomy with Neurotropic Virus | ΔTK-TT |
| SAD-ΔG-GFP | Dr. Diego V. Bohórquez, Assistant Professor Duke Medicine-GI and Neurobiology | N/A |
| AAV5-EF1a-DIO-hChR2(H134R)-EYFP | Dr. Karl Deisseroth-University of North Carolina’s Vector Core | N/A |
| AAV5-EF1a-DIO-EYFP | Dr. Karl Deisseroth-University of North Carolina’s Vector Core | N/A |
| AAV-hSyn-DIO-rM3D(Gs)-mCherry | Dr. Bryan Roth - University of North Carolina’s Vector Core | N/A |
| AAV5-flex-taCasp3-TEVp | Dr. Nirao Shah - University of North Carolina’s Vector Core | N/A |
| AAV5-CA-FLEX-RG | Dr. Naoshige - UchidaUniversity of North Carolina’s Vector Core | N/A |
| AAV8.2-hEF1a-DIO-synaptophysin-EYFP | Viral Gene Transfer Core, McGovern Institute for Brain Research, Massachusetts Institute of Technology | N/A |
| CAV2-Cre-GFP | Institut de Génétique Moléculaire de Montpellier, France (Junyent and Kremer, 2015) | N/A |
| PRV-GFP | NIH Center for Neuroanatomy with Neurotropic Virus | 152 |
| PRV-RFP | NIH Center for Neuroanatomy with Neurotropic Virus | 614 |
| Software and Algorithms | ||
| Matlab R20 17b | MathWorks | http://www.mathworks.com/products/matlab/ |
| Offline Sorter | Plexon | www.plexon.com/products/offline-sorter |
| PatchMaster 2.20 | HEKA | http://www.heka.com/index.html |
| Igor Pro 6.36 | WaveMetrics | https://www.wavemetrics.com/ |
| EthoVision XT 11.5 | Noldus | http://www.noldus.com/animal-behaviorresearch/products/ethovision-xt |
| LabView 2014 | LabView | http://www.ni.com/download/labviewdevelopment-system-2014/4735/en/ |
| GraphPad Prism 7 | GraphPad | http://www.graphpad.com/scientificsoftware/prism/ |
| Adobe design standard CS6 | Adobe | http://shop.adobe.com/store/adbehme/en_IE/pd/ThemeID.29250800/productID.249257000 |
| SPSS 21.0 | IBM Predictive Software | https://www.ibm.com/support/knowledgecenter/SSLVMB |
| Spike2 version 7 | CED (Cambridge Electronic Design Ltd) | http://ced.co.uk/products/spkovin |
| ImageJ bundled with 64-bit Java 1.8.0_112 | NIH ImageJ | https://imagej.nih.gov/ij/index.html |
| Other | ||
| IntraLipid 20%, emulsion | Sigma | I141–100ML |
| Totally Light 2Go - non-caloric powdered flavor drink mix, cranberry and wild berry flavors; sugar-free, Splenda-sweetened | 4C Foods Corp. | N/A |
| Implantable Optical Fibers | Doric Lenses, Canada | MFC_200/240–0.22_6mm_ZF1.25(G)_FLT |
| Formvar-Insulated Nichrome Wires (EMG recording) | A-M system | Cat # 762000 |
| Male Miniature Pin Connector Fits Model 1800 / 3000 Headstage Leads (EMG recording) | A-M system | Cat # 520200 |
| Female Miniature Pin Connector Fits A-M Systems’ electrodes (EMG recording) | A-M system | Cat # 520100 |
| A-M Systems 1700 AC Amplifier | A-M systems | Cat # 690000 |
| 2mm, cut off 6kDa, Microdialysis Probe and guide cannula | CMA | CMA-7, |
| 16 tungsten microwires, 35-μm diameter (Electrophysiological recordings) | TDT systems | Cat # OMN1005 |
| The minimally traumatic elastic cuff electrodes (Micro Cuff Sling (200 μm/3pol/2,5mm/ cable entry top) | CorTec GmbH | N/A |
| Infra-red pulse oximeter | Starr Life Sciences Corp | MouseOx Plus |
| Collar Clip Sensor | MouseOx Plus Sensor, Starr Life Sciences Corp | SLS-015 021 |
| MATLAB script for electromyogram detection | de Araujo lab | https://www.dropbox.com/sh/6b3y7ult47m8mje/AAANdvXSwj2-iT39AP41kHQ1a?dl=0 |
| MATLAB script for PCA calculation of neuronal data | de Araujo lab | https://www.dropbox.com/sh/ei2o6hdcqp4t8zf/AABnzqg5_eRE9Pp0oo16DfTQa?dl=0 |
| Spike2 scripts to count neurogram and myogram events | de Araujo lab | https://github.com/matthewperkins/WH_IdA_VagusPaper |
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