KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-NK1.1 (clone pk136) | Bio-X-Cell | BE0036; RRID: AB_1107737 |
| Mouse anti-IgG2a isotype control (clone C1.18.4) | Bio-X-Cell | BE0085; RRID: AB_1107771 |
| Mouse anti-CD4 AF700 | Biolegend | 100430; RRID: AB_493699 |
| Mouse anti-CD44 BV510 | Biolegend | 103044; RRID: AB_2650923 |
| Mouse anti-PD-1 ef450 | eBiosciences | 48-9981-82; RRID: AB_2574139 |
| Mouse anti-CXCR5 PE-Cy7 | Biolegend | 145516; RRID: AB_2562209 |
| Mouse anti-CD49d FITC | Biolegend | 103606; RRID: AB_313037 |
| Mouse anti-IgM FITC | Biolegend | 406506; RRID: AB_315056 |
| Mouse anti-IgD FITC | Biolegend | 405704; RRID: AB_315025 |
| Mouse anti-CD95 PE-Cy7 | BD Biosciences | 557653; RRID: AB_396768 |
| Mouse/human anti-B220 AF700 | Biolegend | 103232; RRID: AB_493717 |
| Mouse anti-GL-7 ef450 | eBiosiences | 48-5902-82; RRID: AB_10870775 |
| Mouse anti-IgG1 APC | Biolegend | 406610; RRID:AB_10696420 |
| Mouse anti-IgG3 BV711 | BD Biosciences | 565809 |
| Mouse anti-CD19 BV510 | Biolegend | 115546; RRID: AB_2562136 |
| Bacterial and Virus Strains | ||
| XL-10 Gold Ultracompetent e.coli | Agilent | 200314 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| NP-KLH | Biosearch Technologies | N-5060-25 |
| TRIzol | ThermoFisher Scientific | 15596026 |
| NP-PE | Biosearch Technologies | N-5070-1 |
| NP-4-BSA | Biosearch Technologies | N-5050L-10 |
| NP-20-BSA | Biosearch Technologies | N-5050H-100 |
| LIVE/DEAD Fixable Aqua dead cell stain kit | Life Technologies | L34957 |
| GoTaq Flexi DNA polymerase | Promega | M8291 |
| Critical Commercial Assays | ||
| Superscript IV reverse transcription kit | Invitrogen | 18090010 |
| GeneJet gel extraction and cleanup kit | QIAGEN | K0691 |
| pGEM-T vector system | Promega | A3600 |
| Qiaprep spin miniprep kit | QIAGEN | 27104 |
| Deposited Data | ||
| Vh186.2 DNA sequences | This paper | https://tf.cchmc.org/external/rydlb5/Rydyznski_SHM_NK_data.zip |
| Experimental Models: Organisms/Strains | ||
| C57BL/6 mice | Jackson Labs | JAX:000664 |
| Oligonucleotides | ||
| CTCTTCTTGGCAGCAACAGC-Forward Primer 1 | This paper | N/A |
| GCTGCTCAGAGTGTAGAGGTC-Reverse Primer 1 | This paper | N/A |
| GTGTCCACTCCCAGGTCCAAC-Forward Primer 2 | This paper | N/A |
| GTTCCAGGTCACTGTCACTG-Reverse Primer 2 | This paper | N/A |
| Software and Algorithms | ||
| pRESTO | Vander Heiden et al., 2014 | https://prestodb.io/ |
| Change-O | Gupta et al., 2015 | https://changeo.readthedocs.io/en/version-0.4.1—airr-standards/ |
| R | R Development Core Team, 2014 | https://www.r-project.org/ |
| NCBI IgBlast package | Ye et al., 2013 | https://www.ncbi.nlm.nih.gov/igbalst/ |
| IMGT/GENE-DB | Giudicelli et al., 2005 | https://www.imgt.org/genedb/ |
| Other | ||
| Xgal-IPTG-Ampicillin agar plates | KD Medical | BPL-2310 |