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. Author manuscript; available in PMC: 2018 Oct 17.
Published in final edited form as: Mod Pathol. 2017 Nov 17;31(3):406–417. doi: 10.1038/modpathol.2017.143

Figure 5: Mapping of epithelial and immune components in ductal carcinoma in situ.

Figure 5:

5A. Representative pseudo-H&E images showing 4 cases of ductal carcinoma in situ with local immune responses.

5B. Composite immunofluorescence images for fields shown in A. Markers include HER4, SLC7A5, HER2, pmTOR, CD44v6, CD10 and EGFR.

5C. Cluster IDs mapped back to images allow clear visualization of cells with different patterns of expression. Colors correspond to cluster IDs in Figure 3, and pie charts shown in inset are for the specific field of view - the inner pie shows relative abundance of the 8 epithelial groups, and the outer ring show proportions of different immune populations.

5D. Verification of immune patterns after semi-automated quantification. CD20 positive cells in red, CD4 in green, and CD8 in blue.