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. 2018 Aug 20;10(10):e8888. doi: 10.15252/emmm.201808888

Figure EV1. Parkin protein levels are unaffected in liver while a small decrease is measured in G93A cerebellum.

Figure EV1

  1. Representative Western blot for Parkin and p62 in cerebellum (Cb) homogenates at 130 days.
  2. Quantification indicates that Parkin is decreased in G93A mice relative to Non Tg at 130 days. Protein levels were normalized by β‐actin. Results are expressed as mean ± SEM and percent of Non Tg; n = 8 (four males and four females) mice per group. *P = 0.039 by paired Wilcoxon's test.
  3. Quantification of p62 in the homogenates indicates that p62 is increased in PKO and PKO/G93A mice, independent of SOD1‐G93A expression, at 130 days of age. β‐actin was used for normalization. Results are expressed as mean ± SEM and percent of Non Tg; n = 8 (four males and four females) mice per group. No statistically significant differences were found between Non Tg and G93A by paired Friedman's test with Dunn's correction (P = 0.99). ***P = 0.0001 by paired Student's t‐test (G93A vs. PKO/G93A).
  4. Representative Western blot of Parkin and p62 in liver homogenates at 130 days.
  5. Parkin protein levels were quantified at 130 days. β‐actin was used for normalization. Results are expressed as mean ± SEM and percent of Non Tg; n = 8 (four males and four females) mice per group. No statistically significant differences were found between Non Tg and G93A (P = 0.546 by paired Wilcoxon's test).
  6. Quantification of p62 protein levels in liver at 130 days. Results are expressed as mean ± SEM and percent of Non Tg; n = 8 (four males and four females) mice per group. No statistically significant differences were found between Non Tg and G93A (P = 0.546 by paired Wilcoxon's test). No statistically significant differences were found among the other groups.

Source data are available online for this figure.