Figure 2. Functional siRNA screen identifies glutathione peroxidases as essential enzymes in renal cancer cells.
A) RCC4, A498, UMRC2, 786-O and 769-P cells were transfected with siRNA oligonucleotides targeting 230 different metabolic enzymes, nutrient transporters and metabolic regulators. 96h post transfection, number of remaining cells was determined and used to calculate z-scores. Graph displays mean z-scores across all cell lines and selected genes are highlighted.
B) Table showing z-scores of selected genes in each of the five cell lines.
C) RCC4, A498, 786-O and 769-P cells were transfected with siRNA oligonucleotides targeting MYC, G6PD, DHCR24, GPX3 or GPX4 or a non-targeting control (RF). Cell numbers were determined 96h post transfection. Values represent mean cell number ±SEM (n=3).
*p≤0.05; **p≤0.01; ***p≤0.005; ****p≤0.001 unpaired two-tailed Student’s t-test.