Table 1:
Photo-physical properties of the mutants derived from FR-13. Brightness values in yeast are the mean fluorescence intensity in the cells expressing the mutants as measured by FACS (Figure 4a). Values of λabs and λem are the maximum wavelengths of visible absorption and emission spectra, respectively, τ is the excited state lifetime, φ is the fluorescence quantum yield, and εmax is the extinction coefficient at the absorption peak. Details of photo-physical measurements are described in SI-Sec. 11.
| RFP | λabs (nm) |
λem (nm) |
τ (ns) |
φ | εmax
(M−1 cm−1) |
Molecular Brightness (ε×φ) |
Brightness (in yeast) |
|---|---|---|---|---|---|---|---|
| FusionRed | 575 | 596 | 1.8 | 0.26 | 87,300 | 100 | 100 |
| FR-13 | 571 | 591 | 2.7 | 0.48 | 124,000 | 262 | 27 |
| FR-F | 571 | 591 | 2.6 | 0.36 | 104,000 | 165 | 138 |
| FR-G | 572 | 591 | 2.7 | 0.42 | 105,000 | 194 | 144 |
| FR-H | 572 | 592 | 2.6 | 0.42 | 90,100 | 167 | 142 |