Figure 6.
Flow Cytometry Analyses of UCP1 Expression in Mouse White and Brown Adipocytes
(A) Representative images of unsorted BAT adipocytes from untreated or CL 316,243-treated WT or UCP1-KO mice stained with isotype control or anti-UCP1 antibody (red). Intact cells are co-stained with Syto-12 (green). Note the complete lack of UCP1 staining in the knockout sample. Scale bars, 50 μm.
(B) Flow cytometry analysis of BAT (top) and scWAT (bottom) adipocytes from untreated or CL 316,243-treated WT or UCP1-KO mice stained with isotype control or anti-UCP1 antibody.
(C) Representative images of UCP1+ adipocytes (red, SYTO-12 in green) sorted from BAT and scWAT of CL 316,243-treated WT mice. Scale bars, 50 μm. n = 3 mice for each treatment and genotype. See also Figure S6.