Figure 2. Depletion/inhibition of MMPs induce different migratory phenotypes at low and high cell densities.
(A–D) Trajectories of wild type and MMP 9 knockdown HT1080 fibrosarcoma cells at low and high cell densities. (E) Depletion of MMPs using shRNA mediated interference demonstrates different migratory phenotypes at low and high cell density. (F, G) MMPs that specific Matrix Metalloproteinase Inhibitors (MMPIs) target. (H–K) Treatment of fibrosarcoma cells with MMPIs demonstrates different migratory phenotypes at low and high cell density. (L, M) Treatment of carcinoma cells with MMPIs demonstrate different migratory phenotypes at low and high cell density. In all panels, data is represented as mean ± s.e.m from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001(ANOVA) (n = 3).