| Component | Amount (µL) |
| PBS | 1 |
| crRNA:tracrRNA duplex (ex. gRNA1) | 1 (100 pmol) |
| crRNA:tracrRNA duplex (ex. gRNA2) | 1 (100 pmol) |
| Alt-R Cas9 endonuclease | 2 |
| Total volume | 5 µL |
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