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. Author manuscript; available in PMC: 2018 Aug 13.
Published in final edited form as: Methods Mol Biol. 2018;1665:317–340. doi: 10.1007/978-1-4939-7271-5_17

Figure 8.

Figure 8

LacI titration for a 400 bp OS-O1 loop. Markers (+, red online) indicate the average looping probability at each concentration. Black boxes indicate ±1.96*SEMW confidence limits as determined using Eq. 16. Error bars (blue online) indicated weighted standard deviation defined by Eq 15. The dashed line corresponds to linear fit of data (Eq. 2) to estimate Jloop and K1. A minimum of 27 tethers were measured at each concentration, with each particle being tracked for an average of 1400 sec.