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. Author manuscript; available in PMC: 2018 Aug 13.
Published in final edited form as: Methods Mol Biol. 2018;1665:317–340. doi: 10.1007/978-1-4939-7271-5_17

Figure 5.

Figure 5

The XY trajectory of the tethered particle shown in Figure 3 after drift correction. (A) Two-dimensional histogram of particle positions. Shades indicate bin counts (colored online); contours delineate constant height profiles of the distribution. The standard deviations along x and y (σx, σy) are almost identical for this particle attached to a single tether. The value of a symmetry indicator, λ1∕λ1, near unity indicates highly symmetric excursions. (B) X and Y, positions vs. time trajectories for the tethered bead after drift subtraction (black dots); the thick dots (red online) indicate the (unaveraged) trajectory of a reference particle after subtracting drift.