Fig. 2.
Absence of TLR4 protects from the influence of LPS on adaptive thermogenesis, WAT browning, and mitochondrial integrity. a Body weight changes over the course of administering either CL or LPS+CL to the Tlr4-null male mice (n = 6–8). b Representative images of H&E staining for sWAT of TLR4 KO mice (scale bar = 100 µm). c and d Relative gene expression analysis by qPCR of sWAT obtained from TLR4 KO mice received either CL or LPS+CL. e Relative mtDNA contents in axillary sWAT of LPS and LPS+CL-injected TLR4 KO mice. f OCR measurements by Seahorse extracellular analyzer for TLR4-deficient primary mouse adipocytes differentiated from subcutaneous fat-derived stem cell from TLR-deficient male mice, and treated with either vehicle or LPS. Arrow indicates the addition of respiratory inhibitors of oligomycin (Oligo), FCCP, and rotenone plus antimycin A (Rot+AA) (two-way ANOVA, P > 0.05). All data are presented as mean ± SEM. *P < 0.05 and **P < 0.01 by Student’s t test. ns, not significant; nd, not detected.