Fig. 4.
Fatty acid amide hydrolase (FAAH) pharmacological inhibition prevents leptin-mediated effects on body weight gain and food intake following an overnight fast. (A and B) Leptin significantly lowered body weight gain assessed at 12 h and 36 h following refeeding after an overnight fast exclusively in control animals. (B) By contrast, pretreatment with an FAAH inhibitor prevented the leptin-mediated body weight gain effect. (C and D) Similarly, leptin lowered cumulative food intake measured following refeeding after an overnight fast (C) whereas pretreatment with the FAAH inhibitor prevented leptin’s effect on food intake (D). n = 4 to 6 per treatment condition for the measurements of body weight and food intake. Data are presented as means ± SEM. Asterisks denote significant leptin effect. *P < 0.05, ***P < 0.001.