Figure 4.
Apoptosis induction of NSC 95397 in human colon cancer. (A) Flow cytometric analysis of Annexin V/7-AAD staining in SW480, SW620, and DLD-1 cells treated with indicated concentrations of NSC 95397 for 24 h. Annexin V and 7-AAD negative were designated as live cells in the lower left quadrant; Annexin V positive and 7-AAD negative as early apoptotic cells in the lower right quadrant; Annexin V positive and 7-AAD positive as late apoptotic cells in the upper right quadrant. (B) Quantitative analysis of Annexin V/7-AAD staining; (C) representative Western blots showing expression of cleaved caspase-9, caspase-9, cleaved caspase-3, caspase-3, cleaved caspase-7, caspase-7, cleaved PARP, and PARP in SW480, SW620, and DLD-1 cells treated with 20 μM NSC 95397 for 24 h, with actin as loading control. (D) Quantitative analysis of the relative protein expression of cleaved caspase-9, cleaved caspase-3, cleaved caspase-7, and cleaved PARP normalized actin. Values (means + SD) are normalized to actin loading control; * p < 0.05; and ** p < 0.01 using paired t-tests.