Figure 1.
Recycling of vpTR and the TR is not affected by GLUT4 expression. (A) Cells expressing HA-GLUT4-GFP and either vpTR or the TR were loaded to steady state with 125I-Tf and allowed to efflux for times up to 10 min, with or without insulin. The recycling rate constant of the TR or vpTR was calculated as the slope of the natural log of the percentage of radioactivity cell associated versus time. Each bar represents the average of two experiments. Error bars show the difference between the two. (B) The ratio of the recycling rates (insulin/basal) is plotted for the TR and vpTR, for the experiments shown in A.