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Separation of endosomal and Golgi-targeting determinants. HeLa cells transfected with chimera 38–107 (A and B), chimera 89–175 (C and D), or chimera 176–248 (E and F) were cycloheximide treated, fixed, and double stained with the use of anti-HA (A, C, and E) and anti-giantin (B, D, and F). Bar, 10 μm.
