Figure 6. Comparison of the RfaH-opsEC with an Spt5-RNAPII-EC.
A. (top) Schematic showing RfaH domains (NGN, magenta; KOW, orange), with amino acid residues numbered.
(bottom) overall view of the RfaH-opsEC. The RNAP is shown as a molecular surface, the nucleic acids in CPK format (colored as shown in the key or as labeled). RfaH is shown as a backbone ribbon with a transparent molecular surface, colored as in the schematic above. The RfaH-KOW binds directly above the upstream DNA duplex and next to the β flap/wall.
B. (top) Schematic showing the domain organization of Spt5 (Bernecky et al., 2017), with amino acid residues numbered and disordered regions denoted as a dashed line.
(bottom) overall view of the human Spt5-RNAPII-EC (Bernecky et al., 2017). This structure also includes Spt4, which has been removed for clarity. The RNAP is shown as a molecular surface, the nucleic acids in CPK format (colored as shown in the key or as labeled). Spt5 is shown as a backbone ribbon with a transparent molecular surface, colored as in the schematic above. The Spt5-KOW1-L1 domain binds directly above the upstream DNA duplex and next to the Rbp2 flap/wall. Also see (Ehara et al., 2017).
See also Figure S8.