Figure 2.
Dextran sulfate sodium (DSS) colitis in C57BL/6 mice and Smox−/− mice. Animals were treated with 2.5% DSS for 5 days and then kept for 5 more days. (A) Survival was monitored daily; the Kaplan–Meier plot was performed from two separate experiments [n = 18 wild-type (WT) and n = 20 Smox−/− mice]. (B) Colons were harvested, measured, washed, and weighed. (C) Body weights of all the animals in panel (A) were measured daily and are presented as percentage of initial body weight. (D,E) Colons were Swiss-rolled and stained with hematoxylin and eosin (D) and scored for histologic injury (E). The histologic injury score of untreated WT and Smox−/− mice was 0 for both. In panel (E), the green points represent the animals depicted in panel (D). Scale bar, 50 µm. In all panels, ****P < 0.0001 compared with uninfected animals; §P < 0.05, §§P < 0.01, §§§P < 0.001, and §§§§P < 0.0001 versus DSS-treated WT mice.