Figure 6. AMIGO2 Expression Is Controlled by BETs and AP1/TEAD TFs.
(A) Capture of the UCSC genome browser (GRCh37/hg19) showing 800 kb region on human chromosome 12 (y axis = reads per kilobase per million reads). Ensembl genes (blue) and AMIGO2 SEs (red) are shown at the top. Putative TAD/LAD domain is shown at the bottom.
(B) Zoom-in of the AMIGO2 locus showing 40 kb. Five TEs, E1–E5 (red), within the AMIGO2 SE are shown (bottom).
(C) Approximately 60 kb region around the AMIGO2 SE. ChIP-seq was performed for H3K27ac for seven additional melanoma cell lines (black) and four melanoma STCs (green) (Kaufman et al., 2016; Verfaillie et al., 2015). AMIGO2 SE is shown at the bottom (red).
(D) Pearson correlation of number of H3K27ac reads within the AMIGO2 SE and AMIGO2 Log2FPKM (Table S1).
(E) AMIGO2 qRT-PCR in SKmel147 cells following depletion of BRD2, BRD3, or BRD4. Data are represented as mean ± SEM and normalized to PPIA.
(F) AMIGO2 locus as in (B). SKmel147 RNA-seq JQ1(−)/JQ1(+) 6 hr is shown at top.
(G) AMIGO2 locus as in (B).
(H) FOSL2 (left), TEAD4 (right), and AMIGO2 immunoblots of SKmel147 cells 96 hr post-transduction with shSCR or shFOSL2 (shF2 #1 and #2), and shSCR or shTEAD4+TEAD1 (shT4 #1+shT1 #1 and shT4 #2+shT1 #1). HSP90 and GAPDH were used as loading controls.
See also Figure S6.