Table 2. Summary and evaluation of secreted miRNAs by qPCR panels.
| Preliminary screening | Final screening | |||
| conditions | Q | SIPS | Q | SIPS |
| biological replicates (HDF) | N = 1 | N = 1 | N = 3 | N = 3 |
| Time points (days post treatment) | D21 | D21 | D7; D21 | D7; D21 |
| number of cells used for sEV-RNA (average) | 1.27E+07 | 9.13E+05 | 1,50E+07 ± 33% | 1,65E+06 ± 30% |
| screened miRNAs (Exiqon) | 752 | 375 | ||
| detected miRNAs in 2 conditions (average) | 386 | 371 | ||
| detected miRNAs in 1 condition (average) | 156 | 0 | ||
| not detected (average) | 210 | 4 | ||
| raw Ct-values normalized to number of cells used for sEV-RNA | ||||
| detected miRNAs Ct(Average) ≤ 31 | 101 | 187 | 112 | 220 |
| detected miRNAs Ct(Average) 31 - 35 | 197 | 142 | 138 | 132 |
| detected miRNAs Ct(Average) 35 - 38 | 459 | 313 | 121 | 19 |
| detected miRNAs Ct(Average) > 38 | 366 | 235 | 0 | 0 |
| Dataset of miRNAs for statistic quantification (Ct(Average) < 38) | 353 | |||
| miRNAs with complete dataset for 3 Donor D7/D21 | 280/290 | |||
| miRNAs with complete dataset for 2 Donor D7/D21 | 36/38 | |||
| miRNAs with complete dataset for 1 Donor D7/D21 | 36/24 | |||
Preliminary screening of secreted miRNAs to determine detectable miRNAs in small EVs derived from HDFs, was performed using one HDF strain in both conditions, stress-induced premature senescent (SIPS) and quiescent control (Q), from one time point (D21). 375 miRNAs out of 752 screened were selected for the final screening with three different HDF cell strains (n = 3), in 2 conditions (SIPS and Q) and from two time points at 7 (D7) and 21 days (D21) after treatment.