Fig. 1. NOS2 deletion reduces lung SCC development.
a Comparison of lung SCC incidence in KA/KA and KA/KA;Nos2−/− mice at 8 months of age and mouse survival at 12 months of age. P-value between KA/KA and KA/KA;Nos2−/− groups is analyzed by chi-square test. b Western blotting shows TRIM29 and COX2 levels in WT, KA/KA, and KA/KA;Nos2−/− lungs. β-actin, protein loading control. c K5 immunohistochemistry (IHC) stains lung SCCs from KA/KA and KA/KA;Nos2−/− mice. K5 keratin 5; dark brown, positive staining. Scale bar, 50 μm. d The appearances of lungs of KA/KA and KA/KA;Nos2−/− mice. An arrow indicates a lung SCC. e Hematoxylin and Eosin (H&E) examination of lungs of WT, KA/KA, and KA/KA;Nos2−/− mice. Scale bar, 40 μm