Figure 5.
Immunofluorescence of AIRE protein in wild-type (WT) medullary thymic epithelial cell (mTEC) 3.10 cells before and after thymocyte adhesion (A,B) or in mTEC 3.10E6 mutant clone (C,D). Thymocyte adhesion increased AIRE protein nuclear localization in WT mTECs while the mTEC 3.10E6 mutant clone did not exhibited nuclear AIRE protein staining neither before of after thymocyte adhesion. Cells were labeled with an antibody against Mus musculus AIRE protein (red dots), with phalloidin to show the cytoplasmic region and with DAPI to show nuclei (blue).