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. 2018 Mar 22;2018:3734250. doi: 10.1155/2018/3734250

Table 1.

Primer sequences used for MS-HRM analysis. LINE-1-rv contains an additional 5′ biotin residue for possible pyrosequencing use. Primer sequences were taken from literature as indicated. Amplicon length and CpG content were determined using BiSearch version 2.53 [65] and Ensembl release 86 database [66].

Primer Sequence (5′-3′) for MS-HRM Reference Amplicon (bp) CpGs
MLH1-fw TTTTTTTAGGAGTGAAGGAGG [67] 123 13
MLH1-rv AACRCCACTACRAAACTAAA
DNMT1-fw GGTATCGTGTTTATTTTTTAGTAA [68] 115 6
DNMT1-rv ACGAAACCAACCATACCCAA
LINE-1-fw TTTTGAGTTAGGTGTGGGATATA [69] ~143–148 ~1–10
LINE-1-rv AAAATCAAAAAATTCCCTTTC