Figure 3. Single cell lineage analysis of adult organs reveals hierarchies of cell fate decisions.
(a) t-SNE representations of scRNA-seq data for dissociated organs from adult zebrafish (red: heart, green: pancreas + liver, blue: telencephalon; n=3 animals). (b) Lineage tree for organs from one adult. Pie charts are plotted small for n<50, medium for n≥50, and large for n≥1000. Scars with creation probability ≥0.01 were excluded from the analysis. Color code as in (a). (c) Lineage tree zoomed into immune cell types from same adult as (b) (see color code). As expected, immune cells from different organs cluster together in the lineage tree, even though the sequencing libraries for the different organs were prepared separately. This observation is an additional important validation of the scar filtering pipeline, since it shows that even small cell populations such as these immune cells do not acquire scars from other cells types in their organ of origin by mechanisms such as cell doublets or sequencing errors.