Extended Data Table 1.
X-ray statistics of the PHF1 and MTF2 Tudor-PHD1-PHD2-EH cassettes in the free or DNA and/or histone bound states.
| Data collection and refinement statistics | |||||
|---|---|---|---|---|---|
| Crystal | Free human PHF1 (26- 340) form 1 | Free human PHF1 (26-340) form 2 | Human PHF1 (26- 360) and DNA complex | Human MTF2 (42- 358) with DNA and H3(33-40)K36me3 | Mouse PHF1 (26-360) with DNA and H3(29- 41)K36me3 |
| Beam line | APS-19ID | APS-19ID | SSRF-BL18U1 | SSRF-BL19U1 | SSRF-BL19U1 |
| Wavelength | 0.97918 | 0.97918 | 0.97791 | 0.97853 | 0.97852 |
| Space group | P212121 | P212121 | P212121 | P312 | C2 |
| Unit cell a, b, c (Å) | 40.0, 62.0, 135.4 | 61.5,66.8,76.2 | 109.6, 110.3, 118.9 | 137.7, 137.7, 101.2 | 141.2, 62.6, 97.3 |
| Unit cell α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 120.0 | 90.0, 108.0, 90.0 |
| Resolution (Å) | 36.49-1.90 (1.96-1.90)a | 29.34-1.90 (1.96-1.90) | 50.0-2.30 (2.34-2.30) | 50.0-2.25 (2.30-2.25) | 50.0-2.40 (2.46-2.40) |
| Rsym | 0.135 (0.849) | 0.132 (0.807) | 0.123 (0.820) | 0.124 (0.894) | 0.086 (0.485) |
| I/σ (I) | 16.6 (1.9) | 18.8 (2.5) | 22.4 (2.0) | 19.6 (3.0) | 17.9 (1.8) |
| Completeness (%) | 97.3 (96.7) | 100 (100) | 99.9 (100) | 100 (100) | 99.1 (99.1) |
| Redundancy | 4.7 (4.3) | 6.6 (6.1) | 9.2 (9.3) | 16.9 (17.4) | 3.6 (3.3) |
| Unique reflections | 126328 | 171303 | 65188 | 52840 | 32456 |
| Rwork/Rfree (%) | 18.3/21.8 | 17.7/22.7 | 20.9/24.4 | 20.1/23.1 | 22.2/25.7 |
| Number of non-H atoms | |||||
| Protein | 2397 | 2509 | 7290 | 4979 | 5031 |
| DNA | 0 | 0 | 526 | 526 | 506 |
| Water | 183 | 203 | 190 | 331 | 56 |
| ligands | 4 | 4 | 28 | 8 | 8 |
| Average B factors (Å2) | |||||
| Protein | 27.9 | 24.7 | 62.1 | 38.9 | 57.8 |
| DNA | no | no | 58.6 | 41.0 | 72.1 |
| Water | 18.5 | 21.0 | 50.1 | 38.4 | 51.4 |
| Other ligands | 32.4 | 31.2 | 24.5 | 38.6 | 59.6 |
| R.m.s. deviations | |||||
| Bond lengths (Å) | 0.007 | 0.007 | 0.003 | 0.003 | 0.005 |
| Bond angles (°) | 0.998 | 1.04 | 0.641 | 0.634 | 0.793 |
a
Highest resolution shell (in Å) shown in parentheses.