Figure 2. Functional classification and pathway analysis of siRNA screen results.
(A) Pie graph showing functional classification of candidate genes based on Gene Ontology analyses. (B) An overview of enriched pathways generated by Reactome Skypainter analysis. The number of identified genes in each signaling event ranging from 1 to 11 was colored from blue to red accordingly. Enriched reaction groups were highlighted in pink box frames. (C) Negative log [P values] of enriched signaling events. Number of identified genes versus total number of genes in each particular event was listed. (D) Two major signaling networks generated using Ingenuity Pathway Analysis. Screen-identified candidate genes are highlighted in green. Orange dashed circles contain groups of genes involved in mRNA processing; blue dashed circle encompasses a subset of genes related to estrogen receptor signaling.