Extended Data Figure 2. TAM characterization.
a. No primary control for IF images is shown. Cytospinned TAMs were stained with fluorescently-conjugated secondary only (20× magnification, Scale bar=20 μm. Red=594, Green=488, Blue=Hoechst). b. Mouse PD-1− TAMs trend towards an M1 (CD206−MHCIIhigh) expression profile, rather than M2 (CD206+MHC IIlow/neg). TAMs that did not adhere to either of these expression profiles were not classified as M1 vs M2 (n=5. Paired one-tailed t-test). c. Human PD-1- TAMs are predominantly M1 (CD206−CD64+) rather than M2 (CD206+CD64−) (n=10. Paired one-tailed t-test). d. In mice, comparing tumor volume vs. % PD-1+ TAMs shows a highly significant correlation between tumor size and PD-1 expression (n=20. Exponential growth equation is shown). e. Donor chimerism 6-weeks post B.M. transplant. [Granulocytes (Gr1high), 99%; myeloid (CD11b+), 92%; B cell (CD19+), 97%; T cell (TCRβ+), 74%.] *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; n.s., not significant. Data are mean ± s.e.m.