Figure 3. GMP-grade production of MSC-derived exosomes.
(A) The number of exosomes, quantified by NanoSight, produced by 6 consecutive 48-hour isolations (harvests) of MSC-conditioned media from the bioreactor. (B) Particle size distribution analysis using NanoSight. (C) Correlation between the number of exosomes and exosomal protein from the bioreactor harvests (Pearson correlation test). Data shown in A–C are representative of the same data, obtained from the same bioreactor experiment (Bioreactor run 1) (see also Supplemental Figure 5A). (D) Representative TEM of exosomes from each of the 6 consecutive bioreactor harvests. Scale bar: 100 nm. (E) Representative histogram of flow cytometry analysis of exosomal markers (CD9, CD63, CD81) and CD47 on exosomes from bioreactor harvests 1 and 6 (see also Supplemental Figure 5D). Numbers represent the percentage of positive beads (gray, isotype control). See Supplemental Source Data 1 and 2.